ORFeome Collaboration Library


The ORFeome Collaboration Libraries contain fully sequenced, genome-scale human and mouse open reading frame (ORF) libraries cloned into Gateway-entry vectors.
The ORFeome Collaboration (OC) is a group of academic and commercial laboratories formed to develop an unrestricted source of sequence-validated human ORF clones. Using MGC cDNA clones as starting material, the OC has been able to create over 10,000 full-length human ORF clones, promoting the goal of providing at least one ORF for each of the ~18,500 defined human genes. Dharmacon is a major distributor for the OC, providing individual clones, gene family libraries, and whole-genome collections to academic and commercial customers.

Using Gateway recombinational cloning system in all of the OC ORF clones allows for efficient transfer of the ORFs from one vector to another, providing a broad choice of destination vectors. OC ORFs are available with or without native stop codons, providing options for native and fusion-ready protein expression.

Highlights

  • Fully-sequenced, genome-scale ORF collection
  • Easily transferable into any Gateway-adapted expression vector
  • Available with and without stop codons
ORFeome Collaboration - Serving the research community with Human ORF clones

Note

We provide certain clone resources developed by leading academic laboratories. Many of these resources address the needs of specialized research communities not served by other commercial entities. In order to provide these as a public resource, we depend on the contributing academic laboratories for quality control.

Therefore, these are distributed in the format provided by the contributing institution "as is" with no additional product validation or guarantee. We are not responsible for any errors or performance issues. Additional information can be found in the product manual as well as in associated published articles (if available). Alternatively, the source academic institution can be contacted directly for troubleshooting.

Individual Clones

Clones are provided as a live culture in a 2 mL tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. We will ship your clone at room temperature via express delivery. Store the stock clone at -80°C.

Bulk Orders

Orders of 50 clones or greater and stock plates will be provided in microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.

Entire Collection

Orders for the entire C. elegans Promoter collection are provided in 96-well microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for estimated shipping time on orders for the entire collection.

  
HazardousNo
Shipping ConditionDry Ice
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-80 C

References

  1. J. F. Rual et al., Human ORFeome version 1.1: a platform for reverse proteomics. Genome Res. 14, 2128-2135 (2004).
  2. P. Lamesch et al., hORFeome v3.1: A Resource of Human Open Reading Frames Covering over 10,000 Human Genes. Genomics. 89(3), 307-315 (Epub 5 January 2007, March 2007). [In preparation]