ORFeome Collaboration Clones


ORFeome Collaboration Clones are fully-sequenced human open reading frames (ORFs) subcloned into Gateway-entry vectors and are available with or without native stop codons.

Enter a common gene identifier and click SEARCH to find products for your gene.

 
ORFeome Collaboration Mouse Clone
OMM4760 glycerol stock - $373.00select
ORFeome Collaboration Human Clone (without native stop codon) glycerol
OHS5894 glycerol stock - $372.00select
ORFeome Collaboration Human Clone (with native stop codon) glycerol
OHS5893 glycerol stock - $372.00select
ORFeome Collaboration Mouse Clone (without native stop codon) glycerol
OMM5896 glycerol stock - $373.00select
ORFeome Collaboration Mouse Clone (with native stop codon) glycerol
OMM5895 glycerol stock - $373.00select

The ORFeome Collaboration (OC) is a group of academic and commercial laboratories that was formed to develop an unrestricted source of sequence-validated human open reading frame (ORF) clones. This collaboration of commercial and academic researchers has created a collection of fully-sequenced ORFs that will represent all curated genes in the human genome. These clones are verified to have a full-length ORF and are easily transferable into any Gateway-adapted expression vector. Additional clones for this expanding collection are offered as they become available.

Dharmacon is a major distributor for the OC, providing individual clones, gene family libraries and whole-genome collections to academic and commercial customers.

Using the Mammalian Gene Collection (MGC) cDNA clones as starting material, the OC has been able to create over 10,000 full-length human ORF clones, making significant progress toward the goal of providing at least one ORF for each of the ~18,500 defined human genes.

Highlights:

  • Fully-sequenced human open reading frames
  • Gateway-adapted ORFs allow for efficient transfer into a variety of compatible expression vectors
  • Inclusion of native stop codons allows for expression of native proteins
  • Removal of stop codons allows for expression of C-terminal fusion proteins
ORFeome Collaboration - Serving the research community with Human ORF clones

Note

We provide certain clone resources developed by leading academic laboratories. Many of these resources address the needs of specialized research communities not served by other commercial entities. In order to provide these as a public resource, we depend on the contributing academic laboratories for quality control.

Therefore, these are distributed in the format provided by the contributing institution "as is" with no additional product validation or guarantee. We are not responsible for any errors or performance issues. Additional information can be found in the product manual as well as in associated published articles (if available). Alternatively, the source academic institution can be contacted directly for troubleshooting.

Individual Clones

Clones are provided as a live culture in a 2 mL tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. We will ship your clone at room temperature via express delivery. Store the stock clone at -80°C.

Bulk Orders

Orders of 50 clones or greater and stock plates will be provided in microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.

Entire Collection

Orders for the entire C. elegans Promoter collection are provided in 96-well microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at -80°C immediately upon receipt. Please contact our customer service department for estimated shipping time on orders for the entire collection.

  
HazardousNo
Shipping ConditionFrozen Gel Packs,Dry Ice
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-80 C

References

  1. J. F. Rual et al., Human ORFeome version 1.1: a platform for reverse proteomics. Genome Res. 14, 2128-2135 (2004).
  2. P. Lamesch et al., hORFeome v3.1: A Resource of Human Open Reading Frames Covering over 10,000 Human Genes. Genomics. 89(3), 307-315 (Epub 5 January 2007, March 2007). [In preparation]