The ORFeome Collaboration (OC) is a group of academic and commercial laboratories that was formed to develop an unrestricted source of sequence-validated human open reading frame (ORF) clones. This collaboration of commercial and academic researchers has created a collection of fully-sequenced ORFs that will represent all curated genes in the human genome. These clones are verified to have a full-length ORF and are easily transferable into any Gateway-adapted expression vector. Additional clones for this expanding collection are offered as they become available.
Dharmacon is a major distributor for the OC, providing individual clones, gene family libraries and whole-genome collections to academic and commercial customers.
Using the Mammalian Gene Collection (MGC) cDNA clones as starting material, the OC has been able to create over 10,000 full-length human ORF clones, making significant progress toward the goal of providing at least one ORF for each of the ~18,500 defined human genes.
Fully-sequenced human open reading frames
Gateway-adapted ORFs allow for efficient transfer into a variety of compatible expression vectors
Inclusion of native stop codons allows for expression of native proteins
Removal of stop codons allows for expression of C-terminal fusion proteins
Dharmacon is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Technical Support.
Clones are provided as a live culture in a 2mL tube. Each tube contains LB medium supplemented with 8%glycerol and the appropriate antibiotic. Within 2 to 3 business days of receiving your order, we will ship your clone at room temperature via express delivery. Store the stock clone at –80°C.
Orders of 50 clones or greater and stock plates will be provided in 96-well microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at –80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.
- J. F. Rual et al., Human ORFeome version 1.1: a platform for reverse proteomics. Genome Res. 14, 2128-2135 (2004).
- P. Lamesch et al., hORFeome v3.1: A Resource of Human Open Reading Frames Covering over 10,000 Human Genes. Genomics. 89(3), 307-315 (Epub 5 January 2007, March 2007). [In preparation]
Product and Technical Manuals