The Mammalian Gene Collection (MGC) is a trans-NIH initiative, started in 2001. Its goal is to provide researchers with unrestricted access to sequence-validated full-length protein-coding (FL-CDS) cDNA clones for human, mouse, and rat genes. In 2005, the project added the bovine cDNAs generated by Genome Canada.
MGC clones were initially obtained by random EST-screening of cDNA libraries. Starting in 2004, MGC used transcript-specific RT-PCR cloning to isolate cDNAs for genes not represented in MGC. Finally, between 2006 and 2009, MGC employed DNA synthesis methods to provide FL-CDS clones for human and mouse genes still absent from the collection. Details on the vectors and methods employed at each stage of the MGC program are provided on the MGC website: Vectors & Method Overviews.