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    cDNAs and ORFs

    cDNAs and open reading frames (ORFs) can be used to overexpress genes-of-interest. We offer mammalian cDNA and ORF collections which are derived from mRNA including 5' and 3' UTRs.

    The cDNA collections are ideal for overexpressing a gene in the context of native regulation, while ORFs have both 5' and 3' UTRs removed providing a shortcut to protein expression. Additionally, we provide cDNA and ORF collections for Yeast, C. elegans, Zebrafish, Xenopus, and E. coli. Choose from individual clones, lentiviral ORFs, and genome-scale libraries.

    These cDNA and ORF collections provide a quick and accessible method for obtaining gene content for many in vitro and cell culture overexpression applications. The coding sequence is cloned into an expression vector under control of a strong promoter and then delivered to cells of interest by plasmid transfection or lentiviral transduction.

    Applications where cDNAs and ORFs are commonly utilized are gene overexpression studies, RNAi rescue experiments, structural analysis, visualization of protein localization, biochemical purification, and identification of interacting proteins through Co-immunoprecipitation.

    Additionally, CRISPR Activation (CRISPRa) is a new overexpression tool that can be used as an alternative to cDNA and ORFs in several applications (See the table below). CRISPRa uses a deactivated version of S.pyogenes Cas9 often tethered to transcriptional activators (i.e. dCas9-VPR) and CRISPRa guide RNA (designed to target near a gene’s transcription start site (TSS)) to then upregulate the transcription of desired genes endogenously. For more information about CRISPRa technology see our applications page and featured articles.

    See the table below to determine which overexpression tool is best for your research.

    Mammalian cDNAs

    Derived from mRNA and including 5’ and 3’ UTRs, our cDNA collections are ideal for overexpressing a gene in the context of native regulation.

    Mammalian ORFs

    With both 5’ and 3’ UTRs removed, open reading frames (ORFs) provide a shortcut to protein expression.

    • Precision LentiORFs

      Precision LentiORFs are expression-ready open reading frames (ORFs) that have been cloned into a lentiviral expression vector with a GFP reporter.

    • ORFeome Collaboration Collection

      ORFeome Collaboration (OC) clones are fully-sequenced ORFs in Gateway recombinational entry vectors. OC clones are available with and without stop codons.

    • CCSB Human ORFeome

      This Gateway adapted human ORF collection is ideal for easily moving thousands of open reading frames into your choice of protein expression systems.

    • Human ORFeome V8.1 Library

      This fully-sequenced collection of Gateway-adapted ORFs represents the latest version of the CCSB Human ORFeome library from Dana-Farber Cancer Institute.

    • CCSB-Broad Lentiviral Expression Library

      A genome-scale library of human ORFS in a lentiviral vector, stop codons have been removed and clones contain a C-terminal V5 tag.

    Non-Mammalian cDNAs and ORFs

    Your complete source for cDNAs, ORFs, knockout strains, promoter collections and other resources for yeast, C. elegans, Zebrafish, Xenopus, and E. coli.

    • Yeast

      Browse our list of yeast resources including Yeast Knockout strains, Tagged-ORF strains, Genomic DNA, cDNA libraries, and additional collections.

    • C. elegans

      C. elegans ORFs, RNAi feeding collections, promoter and transcription factor collections.

    • E. coli

      Includes the E. coli Keio knockout collection, Promoter-GFP fusions for monitoring gene expression, and tagged ORFs for studying protein-protein interactions.

    • Zebrafish

      Full-length, fully-sequenced Zebrafish ZGC and IMAGE clones, as well as Exelixis and NEIBank cDNAs and the Zebrafish IMCB Collection.

    • Xenopus

      Full-length, fully-sequenced Xenopus ORFeome, XGC, and IMAGE cDNA and EST clones.

    cDNA and ORF Libraries

    Pre-defined gene family collections are available for many of our cDNA and ORF products. Choose from whole genome, gene families, or custom libraries.

    • CCSB Human ORFeome Libraries

      The Gateway adapted human Entry ORF libraries are ideal for easily moving entire families of ORFs into your choice of protein expression systems.

    • Precision LentiORFs

      Precision LentiORFs are expression-ready open reading frames (ORFs) that have been cloned into a lentiviral expression vector with a GFP reporter.

    • ORFeome Collaboration Libraries

      ORFeome Collaboration clones are sequence-verified, human open reading frames (ORFs) subcloned into Gateway recombinational entry vectors.

    • Mammalian Gene Collection Libraries

      The mouse and human MGC libraries contain popular gene families in expression-ready and other vector backbones.

    • CCSB-Broad Lentiviral Expression Library

      A genome-scale library of human ORFS in a lentiviral vector, stop codons have been removed and clones contain a C-terminal V5 tag.

    • Human ORFeome V8.1 Library

      This fully-sequenced collection of Gateway-adapted ORFs represents the latest version of the CCSB Human ORFeome library from Dana-Farber Cancer Institute.

    Resources for cDNAs and ORFs