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Pin-point™ Base Editing Platform

All Dharmacon™ Reagents

Gene Editing

Explore Gene Editing CRISPR guide RNA Cas9 nucleases Controls Custom guide RNA Knock-in templates Cherry-pick library tool - clones Cherry-pick library tool - synthetic CRISPR libraries

Gene Knockdown

Explore Gene Knockdown siRNA shRNA microRNA Controls lncRNA Custom siRNA design Cherry-pick library tool - synthetic Cherry-pick library tool - clones RNAi libraries

Gene Expression

Explore Gene Expression CRISPRa cDNA & ORFs

CRISPR Modulation

Explore CRISPR Modulation CRISPRi CRISPRa CRISPRa libraries dCas9 stable cell lines Controls

Reference Standards

Explore Reference Standards Create your own reference standard Oncospan Analysis technique Sample type Format

Libraries

Explore Libraries CRISPR libraries RNAi libraries Cherry-pick library tool - clones Cherry-pick library tool - synthetic

Engineered Cell Models

Explore Engineered Cell Models Knockout cell lines Double knockout cell lines Cancer related cell lines Cas9 stable cell lines dCas9 stable cell lines

Custom Oligonucleotides

Explore Custom Oligonucleotides Single-strand RNA synthesis Custom siRNA Custom antisense oligonucleotides Custom DNA Custom microRNA

Non-mammalian research

Transfection & Ancillary Reagents

Bioproduction Cell Lines

Explore Bioproduction Cell Lines CHOSOURCE™ expression platform CHOSOURCE™ ADCC+ cell line CHOSOURCE™ TnT transposon technology

Functional Genomic Screening

Explore Functional Genomic Screening Arrayed screens Pooled screens CRISPR screens RNAi screens Single-cell CRISPR screens Immune cell screens Pin-point base editing

Immune Cell Screening

Explore Immune Cell Screening CRISPR immune cell screens Custom immune cell screens

Compound screening assays

iTreg Polarization assay iTreg Suppression assay T Cell Activation assay Mixed Lymphocyte Reaction assay

Cell Panel Screening

Explore Cell Panel Screening 2D OncoSignature 2D OncoSignature Long-term assay 3D OncoSignature Custom screens

Cell Line Engineering

Explore Cell Line Engineering Knockout Knock-in Bioproduction cell engineering

Custom Libraries

Custom Oligonucleotides

Contract Research Services

Pin-point™ Base Editing Services

Custom Reference Standards

Gene Editing

Explore Gene Editing Engineering a knockout cell line Synthetic sgRNA for CRISPR-Cas9 experiments CRISPR-Cas9 guide RNA algorithm Inducible lentiviral Cas9 nuclease Homology-directed repair with a DNA donor oligo Homology-directed repair with a plasmid donor Multiplex CRISPR-Cas9 knockout All-in-one CRISPR knockout Synthetic lethality

CRISPRmod

Explore CRISPRmod CRISPR interference CRISPR activation CRISPRa MS2 tracrRNA All-in-one CRISPR modulation

RNA Interference

Explore RNA Interference siRNA Self-delivering siRNA shRNA microRNA Noncoding RNA

Engineered Cell Lines

Explore Engineered Cell Lines Antibody validation Autophagy pathway Bromodomain containing proteins Caspases Deubiquinating enzymes (DUBs) DNA damage pathways Drug targets Epigenetics Histone acetylation iPSCs Kinase inhibition mTOR pathway Parkinson's disease Phosphoinositide function Phospholipases Primary and stem cells Rab GTPases Serine threonine phosphatase Sialylation pathway Solute carrier slc gene Sumoylation TNF alpha Ubiquitin conjugating enzymes

Functional Genomic Screening

Explore Functional Genomic Screening Screening validation and follow-up using engineered cell lines Reverse transfection for high-throughput CRISPR studies C911 controls for siRNA screening Seed sibling controls for RNAi hit validation

cDNA/ORF Overexpression

Custom RNA synthesis

Explore Custom RNA synthesis Antisense oligos

Transfection

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Application notes Exome data Technical manuals Protocols Certificate of analysis Posters Publications Quick protocols Reading lists Safety data sheets Product data White papers

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Education & Events

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On the Horizon Blog

Explore On the Horizon Blog Gene editing Gene modulation Cell models Screening Reference standards CRISPR modulation Custom synthesis Tech Tips

Tools & Calculators

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Biochemical conversion tools

OD conversion DNA copy number calculator

Reagent calculator

siRNA/miRNA calculator for screening DharmaFECT calculator for screening

BioIT beta tools

P-value to Z-score converter T7EI calculator nmol to µg calculator

Primer design & Analysis tools

Multiple primer analyzer TM calculator

qPCR

qpcr efficiency calculator

FAQs

Vector maps & sequences

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Products & Services FAQs

Explore Products & Services FAQs Gene editing Screening Cell lines Reference standards Custom Synthesis Transfection & transduction Viral packaging

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Custom Ordering Tools

Explore Custom Ordering Tools CRISPR design tool HDR donor design Custom siRNA siDESIGN center Cherry-pick libraries Custom microRNA Custom SMARTpool ssRNA synthesis Custom DNA

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Gene Landing Page

LOC100490741.L( African Clawed Frog )

Entrez Gene 431932 Entrez Gene 431932

E3 ubiquitin/ISG15 ligase TRIM25-like L homeolog

Alias

trim25

Product Category

Non Mammalian

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