Considerations for successful knockout cell line generation
CRISPR-Cas9 has made it easier and faster to create an engineered cell line, giving rise to the popularity of the approach in a number of experimental areas, such as protein-protein interaction, hit validation, genomic functional analysis, and creation of disease models. Applications such as protein tagging also help enrich our understanding of particular gene targets. To precisely edit or knockout a genomic target, there are several considerations to be taken into account. Our Cell line engineering webinar goes into these topics in detail, here is a quick look at what it covers:
If you would like to save the time and effort involved in doing your own cell line engineering, you should review the catalog of ready-made cell lines available from Horizon Discovery. The catalog contains our proprietary HAP1 cell lines, plus an expanding range of edited cell lines that include common cancer cell lines such as DLD1, MCF10a and HCT116.
The available knockout cell lines have complete loss of function for the target gene, and knock-in cell lines can improve understanding of a disease gene’s role in a phenotype from relevant mutations.
Authors: Louise Baskin, Senior Product Manager
Powerful cell line tools for applications such as pathway analysis or gene regulation assays to save researchers the time and resources of editing and characterizing clonal cell lines.
Tools to create your own edited cell line.
CRISPR Guide RNA
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