Design a single-strand RNA molecule with a wide variety of chemical modifications, learn about our capabilities for long RNA oligos, dye labeling, custom amidites and more.
Learn about customizing your siRNA with proprietary modification patterns or other chemical modifications, investigate your options for in vivo RNAi, design your own siRNA using the siDESIGN Center, learn about custom SMARTpool options and more.
Customize a miRIDIAN Mimic or Hairpin Inhibitor, or design your own microRNA reagent with specialized chemical modifications, in vivo processing, gram quantities and more.
RNA applications can be executed with greater ease and confidence by utilizing the powerful advantages of the 2'-ACE technology.
Download the Tech Note 2'-ACE RNA Synthesis Chemistry for more information on the details of the RNA synthesis platform.
All synthetic RNA oligos must be chemically protected at multiple sites during synthesis, regardless of the chemistry platform used. However, 2'-ACE synthesis chemistry allows for the quickest and mildest deprotection conditions compared to other chemistries like 2'-tBDMS and 2'-TOM. Short deprotection times and a mild chemical environment promote the highest level of purity for synthetic RNA in the marketplace today.
For unmodified, unpurified RNA, the following approximate percentages of full-length material can be expected:
Unlike other synthesis chemistries, unmodified siRNA or RNA oligos >35 nt synthesized using 2'-ACE chemistry do not require purification prior to in vitro applications.
Comparison of an unmodified, unpurified 36 nt RNA oligo synthesized using 2'-ACE, 2'-tBDMS, and 2'-TOM chemistry platforms. Anion exchange-HPLC traces for (A) 2'-ACE, (B) 2'-tBDMS, and (C) 2'-TOM illustrate a superior level of purity achieved with 2'-ACE chemistry. Smaller peaks eluting earlier than the full-length oligo (shaded area) are indicative of incomplete, non full-length, n-x products. Results are typical, but may vary slightly depending on oligo sequence.
*The average stepwise coupling efficiencies (ASCE) for each method.
**Percent full-length material listed above are approximate; exact yields will depend on sequence composition.
Actual yields obtained for the final product may range from 10%-90% of the scale, depending on the oligo specifications (i.e. modification type, length, purification process, etc.).
If you have a minimum yield requirement for your experiments, please contact Technical Support for recommendations on which scale to select for your online order.
For single-strand RNA, yields may vary greatly depending on the specifications of your request (oligo length, sequence, modifications, purification, ect.). Please contact Technical Support for RNA yield estimates.
*Yields reported above should be considered estimates, and are not guaranteed amounts. Please inquire within if a minimum guaranteed amount is required.
"**Standard processing includes 2'-ACE deprotection, desalting, and annealing.
We pride ourselves on our reputation for high quality RNA synthesis.
See below for definitions
ESI MS: Electrospray ionization mass spectrometryHPLC: High performance liquid chrmatographyPAGE: Polyacrylamide gel electrophoresisUPLC: Ultra performance liquid chromatographyCGE: Capillary gel electrophoresis