Accell siRNA reagents are specially modified for use in difficult-to-transfect cells without the need for transfection reagents, virus, or electroporation. The following selected peer-reviewed publications have cited their successful use in a variety of experimental systems.
Established cell lines
ARPE-19 (human retinal epithelial cells) - 39
BxPC3 (pancreatic tumor cell lines) - 9
C1 tumor derived cells - 52
Caco-2 (colon colorectal adenocarcinoma) - 27
CD4+ primary human T cells - 4, 71
CD14+ primary monocytes - 21, 36
DG-75 human B lymphocytes – 78
GH3 (rat somatolactotrophs pituitary cell line) - 62
H9 stem cell lines - 49
HCT-116 (colorectal carcinoma) - 28
HUVEC - 29
JJN3(plasma cell leukemia) - 44
KG1 (human acute myelogenous leukemia (AML) macrophage cell line) - 68
LUHMES (Lund human mesencephalic cells) - 69
MEC1 (human chronic B cell leukemia) - 14
MN-1 - 35
MS1 (mouse pancreatic islet endothelia cells) – 22
NOD CD4+CD25− splenic cells - 41
NOXA - 44
OVCA 420 (ovarian carcinoma) - 58
PGA-1 (lymphocytic leukemia B cell line) - 67
RAW264.7 macrophages - 55
SHSY5Y (neuroblastoma) - 12, 25, 66
SKBR3 (ER-/PR-/HER2+ breast cancer cell line) - 72
SNB19 glioma cells - 13
T47D (ductal breast epithelial tumor cell line) - 23
T98 glioma cells - 13
THP-1 monocytes - 11, 26, 46, 51, 63
U266 (peripheral blood B lymphocyte myeloma) - 44
U937 (leukemic monocyte lymphoma) - 54
Primary cells & in vivo
β-islet cells - 15
Bone marrow cells - 10, 17
Bronchial smooth muscle cells (BSMC) - 30, 31
Cardiomyocytes - 5
Cerebellar granule neurons (CGN) - 8, 70
Colon stem/progenitor cells - 76
Corneal endothelial cells (adult human CECs), and ex vivo human corneal endothelium - 75
Cortical neurons - 1, 8, 45, 59, 69
Endometrial cells - 16
Endothelial cells - 7, 37
Extravillous trophoblasts (EVT) - 32
Fibroblasts (primary) - 73
Hepatocytes - 40, 42, 50
Immortalized B cells - 65
Keratinocytes - 57
Lung epithelial cells - 80
Lymphocytes - 47
Macrophages - 3, 38, 53
Mantle cell lymphoma cells (MCL) - 48
Monocytes - 19
Mouse embryonic fibroblasts (MEF) - 24
Naïve fetal T cells - 82
Natural killer (NK) cell line - 61
Neonatal mouse ovary - 81
Neurons (primary rat) - 20
Neurons derived from iPS cells - 74
Oligodendrocyte precursors - 60
Pancreatic tumor cell lines - 9
Peripheral blood mononuclear cells (PBMC) - 6, 34, 85
Regulatory T cells - 83
Stem cell–derived peripheral neurons - 79
Vascular smooth muscle cells (VSMC) - 2, 64
In vivo skin delivery - 18
In vivo rat periodontal model - 33
In vivo mouse intradermal injection - 43
In vivo mouse model - 56, 84
In vivo mouse brain - 69
In vivo rat brain - 86, 87, 88