Human Duet siRNA Library - Ubiquitin Conjugation Subset 3

Screen with the two most reliable siRNA reagents available


Paired screening libraries of siGENOME and ON-TARGETplus siRNA for guaranteed silencing of human RING finger E3 ligases.
Duet siRNA Libraries are paired libraries of siGENOME and ON-TARGETplus siRNA SMARTpool reagents. The Human Duet Ubiquitin Conjugation Subset 3 siRNA Library contains siRNAs targeting RING finger and RING finger-like E3 Ligases. These enzymes form a site of interaction with E2s through a platform generated as a consequence of coordination of two zinc ions. U-box E3s conform like RING finger proteins except that they do so through salt bridges and other interactions that do not involve coordination of zinc. A20-like proteins, also included in this set, contain a single coordination site for zinc.

Highlights

  • Increase confidence in your RNAi screen with two guaranteed, highly functional SMARTpool siRNA reagents (see Figure 1, Supporting Data tab)
  • Screen the libraries side-by-side to move high confidence hits through the pipeline faster (see Figure 2, Supporting Data tab)
  • Use the libraries separately as a cost-effective way to obtain two guaranteed collections of highly potent siRNA reagents

siRNA Designs

Both siGENOME and ON-TARGETplus siRNA reagents utilize the same SMARTselection foundational elements for highly functional designs. ON-TARGETplus designs are additionally filtered for specificity-enhancing features that improve the potential for unique designs.

  • In 30% of genes (across the human genome), all eight siRNA sequences targeting a single gene are unique.
  • Only 5% of genes share four siRNA sequences across both product lines.
  • Even in cases of identical designs, it has been widely demonstrated that ON-TARGETplus specificity-enhancing modifications provide distinct and reduced off-target signatures and thus can be treated as unique silencing reagents.

Gene Targets

For a complete list of target genes in these siRNA Libraries, please email Technical Support, or call 1-800-235-9880. International customers, please call 303-604-9499 or your local Sales Representative.

Experimental considerations

For a thorough investigation of the ubiquitin pathway, you may also consider these additional siRNA libraries:

  • Deubiquitinating enzymes
  • Ubiquitin Conjugation Subset 1 - Cullins, E1, E2, HECT E3 Ligases
  • Ubiquitin Conjugation Subset 2 - F-box, SOCS box E3 Ligases
  
HazardousNo
Shipping ConditionAmbient
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-20 C

Our siRNA knockdown guarantee

siGENOME and ON-TARGETplus siRNA reagents (SMARTpool and three of four individual siRNAs) are guaranteed to silence target gene expression by at least 75% at the mRNA level when used under optimal delivery conditions (confirmed using validated positive control and measured at the mRNA level 24 - 48 hours after transfection using 100 nM siRNA).

Note: Most siGENOME and ON-TARGETplus siRNA products are highly functional at 5-25 nM working concentration.

siGENOME and ON-TARGETplus SMARTpool reagents are highly potent

siGENOME and ON-TARGETplus SMARTpool reagents are highly potent

siGENOME and ON-TARGETplus SMARTpool reagents are highly potent

Target mRNA knockdown in a screen with siGENOME and ON-TARGETplus SMARTpool siRNA reagents was highly effective, even at concentrations of 2 nM. In extended studies, ON-TARGETplus also was effective in reducing false phenotypes due to off-targets.


Duet siRNA libraries support high-confidence screening workflows

Duet siRNA libraries support high-confidence screening workflows

Duet siRNA libraries support high-confidence screening workflows

Our products offer more guaranteed siRNA reagents than any other provider to enable the broadest range of siRNA screening strategies. By leveraging the high-efficiency silencing of market-leading siRNA reagents, the execution of a Duet siRNA screen with two unique SMARTpool siRNA reagents (siGENOME and ON-TARGETplus) increases overall primary hit quality and reduces follow-up experimental time and effort.


References

  1. B.D. Parsons, A. Schindler, D.H. Evans, E. Foley, A direct phenotypic comparison of siRNA pools and multiple individual duplexes in a functional assay. PLoS One. 4(12), e8471 (2009).
  2. M. Jiang, R. Instrell, B. Saunders, H. Berven, M. Howell, Tales from an academic RNAi screening facility; FAQs. Brief Funct. Genomics. 10(4), 227-237 (2011). [doi: 10.1093/bfgp/elr016]