Target mRNA knockdown in a screen with siGENOME and ON-TARGETplus SMARTpool siRNA reagents was highly effective, even at concentrations of 2 nM. In extended studies, ON-TARGETplus also was effective in reducing false phenotypes due to off-targets.
Our products offer more guaranteed siRNA reagents than any other provider to enable the broadest range of siRNA screening strategies. By leveraging the high-efficiency silencing of market-leading siRNA reagents, the execution of a Duet siRNA screen with two unique SMARTpool siRNA reagents (siGENOME and ON-TARGETplus) increases overall primary hit quality and reduces follow-up experimental time and effort.