Screen them together or use them separately to support a wide range of RNAi discovery efforts.
Figure 1: Duet siRNA libraries support high-confidence screening workflows. By leveraging the high-efficiency silencing of market-leading siRNA reagents, the execution of a Duet siRNA screen with two SMARTpool siRNA reagents (siGENOME and ON-TARGETplus) increases overall primary hit quality and reduces follow-up experimental time and effort.
Duet siRNA Libraries are paired collections of siGENOME and ON-TARGETplus siRNA SMARTpool reagents, providing knockdown by the two most reliable siRNA reagents available. This unique screening format allow faster
identification of high-confidence hits.
Click on a collection catalog number to view availability and ordering information for that Duet siRNA library.
siGENOME and ON-TARGETplus SMARTpool reagents are highly potent. Target mRNA knockdown in a screen with siGENOME and ON-TARGETplus SMARTpool siRNA reagents was highly effective, even at concentrations of 2 nM. In extended studies, ON-TARGETplus also was effective in reducing false phenotypes due to off-targets.