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    Cherry-pick Clone Libraries

    Enjoy fast and easy online ordering of vector-based reagents – no quote required!

    Choose from our predesigned products lines of lentiviral sgRNA, shRNA, and over-expression reagents to build your own custom library.

    • Our online Library Plater offers the flexibility to select clones and generate a library for your unique experimental needs
    • Choose vector-based reagents from Dharmacon sgRNA, shRNA, or mammalian gene-expression collections; formatted as glycerol stocks. For synthetic reagents, please use Cherry-pick Libraries
    • Select specific clones for a gene or add everything available
    • Clones will automatically be separated onto different plates by antibiotic resistance marker and product format

    Overview

    When Dharmacon pre-defined sgRNA, shRNA, or over-expression libraries do not match your experimental requirements, you can make your own custom clone library with ease, speed and economy.

    You can cherry-pick from our glycerol stock format sgRNA, shRNA, and mammalian over-expression product lines for human and mouse reagents to create the ideal clone collection for your particular needs.

    Here's how:

    cherry-pick clone libraries workflow
    1. Load gene identifiers for your desired products
      • NCBI Gene ID or Gene Symbol
      • Dharmacon product catalog numbers
    2. Select the product type
    3. Review the results for accuracy and product availability
    4. Select the product lines and product formats to include on the plate
    5. Add to cart and check out!

    Cherry-pick Library options

    Use the Library Plater to order these products in 96-well plates
    sgRNA Product Lines Edit-R Lentiviral sgRNA
    shRNA Product Lines GIPZ, TRIPZ, TRC
    Over-expression Product Lines Mammalian Genome Collection (MGC), Precision LentiORFs, CCSB-Broad Lentiviral Expression Library, ORFeome Collaboration Collection, Human ORFeome v8.1 Library, CCSB Human ORFeome
    Product format Glycerol stock
    Species Human or mouse
    Plate type 96 well; 20 well minimum of same product line

    Product Line Selection

    Which shRNA product is right for you?

    Dharmacon offers three complete predesigned product lines across human and mouse genomes. Use the table below to help you in determining the right shRNA product for your needs.

    GIPZ TRIPZ TRC
    Species Human, mouse Human Human, mouse
    Promoter Human CMV (Pol II) TRE min-CMV U6 (Pol II)
    Hairpin format micorRNA-adapted micorRNA-adapted Simple
    Fluorescent reporter TurboGFP TurboRFP None
    Inducible expression No Yes No

    Which over-expression product is right for you?

    Dharmacon offers five different mammalian open reading frame (ORF) collections and the Mammalian Genome Collection (MGC) cDNA collection. Use the table below to help you in determining the right over-expression product for your needs.

    Mammalian Genome Collection (MGC) Precision LentiORF CCSB-Broad Lentiviral Expression ORF ORFeome Collaboration Collection Human ORFeome V8.1 CCSB Human ORFeome
    Format cDNA ORF ORF ORF ORF ORF
    Species Human, Mouse Human Human Human, Mouse Human Human
    Fully Sequenced Yes Yes Yes Yes Yes No
    Expression ready Some Yes Yes No No No
    Subcloning method Restriction digest N/A N/A Gateway® Gateway® Gateway®
    Selection marker N/A GFP V5 epitope tag N/A N/A N/A

    Product Formats Available for Cherry-pick Library Plater

    All products are delivered as arrayed glycerol stocks in 96-well plates

    sgRNA clones

    Select from our predesigned Edit-R Lentiviral sgRNA Druggable Genome collection with up to 4 sgRNA constructs per gene; minimum 20 constructs per order. We recommend choosing 3-4 constructs per gene.

    shRNA clones

    Select from either: GIPZ, TRIPZ, or TRC lentiviral shRNA collections; minimum 20 constructs per order. We recommend selecting 3-5 constructs per gene.

    Over-expression clones

    MGC collection: Cannot be mixed with other collections, minimum 20 constructs per order; clones will be plated according to antibiotic requirements

    ORF collections

    May be combined with other ORF collections to increase coverage for desired library; minimum 20 constructs from a single collection per order. Clones will be plated by collection. (e.g. a customer library of 20 ORFeome collaboration clones and 5 hORFeome v8.1 clones will qualify for library plating, a custom library of 15 ORFeome collaboration clones and 10 hORFeome v8.1 will not)

    Lentiviral Guide RNA

    Investigation of entire gene families or biological pathways is enabled by our broad suite of custom and pre-defined libraries of CRISPR-Cas9 reagents that take advantage of our algorithm-optimized guide RNA designs for high functionality and superior specificity.

    microRNA-adapted shRNAs

    Designed using microRNA scaffold-specific attributes for highly efficient processing via the endogenous RNAi pathway while minimizing off-target potential

    Simple hairpin shRNAs

    Offers a classic hairpin, rules-based shRNA design utilizing a simple hairpin of 21 base pair sense and antisense stem, and a 6 basepair loop

    • TRC Lentiviral shRNA

      Lentiviral shRNA collection from The RNAi Consortium (TRC) with coverage in human and mouse.

    cDNA and ORF Libraries

    Pre-defined gene family collections are available for many of our cDNA and ORF products. Choose the product line that best fits your experimental needs.

    • Mammalian Genome Collection

      The mouse and human MGC libraries contain popular gene families in expression-ready and other vector backbones.

    • Precision LentiORFs

      Expression-ready open reading frames (ORFs) that have been cloned into a lentiviral expression vector with a GFP reporter.

    • CCSB-Broad Lentiviral Expression Library

      Expression-ready human ORFs in a lentiviral vector, stop codons have been removed and clones contain a C-terminal V5 tag.

    • ORFeome Collaboration Libraries

      Sequence-verified human and mouse open reading frames (ORFs) subcloned into Gateway® recombinational entry vectors.

    • Human ORFeome V8.1 Library

      Fully-sequenced collection of Gateway-adapted ORFs representing the latest version of the CCSB Human ORFeome library from Dana-Farber Cancer Institute.

    • CCSB Human ORFeome Libraries

      The Gateway® adapted human Entry ORF libraries, ideal for easily moving entire families of ORFs into your choice of protein expression systems.