miRIDIAN microRNA Mimic Endogenous Positive Control

A validated miRIDIAN microRNA Mimic designed to target Aldolase A in human, mouse, and rat


The miRIDIAN microRNA Mimic Endogenous Positive Control helps you monitor specific mimic effects on target protein levels in a validated endogenous assay, which is based upon the targeted activity of miR-122 on Aldolase A mRNA levels in cell lines.

miRIDIAN microRNA Mimic Positive Controls are ideal for optimizing conditions for relevant, well-controlled microRNA modulation experiments. Both positive and negative control molecules are provided for gain-of-function microRNA studies.

Successful microRNA functional studies begin with optimization of the assay in each cell line and/or type of interest. To optimize experimental conditions, we offer both housekeeping and endogenous positive controls for gain-of-function experiments using miRIDIAN Mimics.

Highlights

  • Validated microRNA Mimic that targets Aldolase A in human, mouse, and rat
  • Same structure and design as experimental miRIDIAN microRNA Mimics
  • Allows optimization of assay conditions by monitoring mimic function on an endogenous gene target (Aldolase A) with a conserved miR-122 binding site (see Figure 1, Supporting Data)
  • Based upon the targeted activity of miR-122 on Aldolase A mRNA levels in cell lines, expressing low to moderate levels of miR-122

Applications

  • Link the activity of a microRNA mimic to post-transcriptional regulation of its putative gene target
  • Measure modulation of endogenous Aldolase A mRNA levels
  
HazardousNo
Shipping ConditionAmbient
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-20 C
miRIDIAN microRNA Mimic Endogenous Control leads to regulation of Aldolase A in human, mouse and rat cell lines

miRIDIAN microRNA Mimic Endogenous Control leads to regulation of Aldolase A in human, mouse and rat cell lines

miRIDIAN microRNA Mimic Endogenous Control leads to regulation of Aldolase A in human, mouse and rat cell lines

Many cells lines express low to moderate levels of miR-122. Aldolase A is a predicted target of miR-122, and the 3' UTR is conserved in human, mouse, and rat at the 8mer miR-122 predicted seed site. miRIDIAN microRNA Mimics designed to modulate endogenous miR-122 was transfected at 50 nM (Huh-7 at 40 nM) using DharmaFECT 1 into the indicated cell lines and assessed for their ability to decrease AldoA mRNA levels. AldoA down-regulation was determined using the Quantigene® branched DNA assay (Panomics) at 3 days (HepG2 at 5 days) post-transfection.


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