Loss-of-function screening on hundreds to tens of thousands of genes at once
Dharmacon Edit-R CRISPR-Cas9 libraries enable you to more easily explore relevant biological questions and examine complex phenotypes in human, mouse, and rat cell lines. All Edit-R guide RNAs are designed using our Edit-R algorithm to give you robust, reliable gene knockout that is highly reproducible across replicates. Libraries are available for specific gene sets, including the entire genome; Cherry-pick libraries are also available to target any custom set of genes. With four guide RNAs per gene in crRNA arrayed libraries, and up to 10 in pooled lentiviral libraries; Edit-R CRISPR-Cas9 screening libraries provide the breadth and depth of coverage you need to be confident in the results you achieve.
Using the discriminatory power of arrayed libraries, screening against one guide RNA per well, you can assay significantly more complex or subtle phenotypes. The use of high-content assays is possible, which will give you deeper insight into your biology. With the ability to directly compare results between the four reagents per gene you can more easily stratify your hits and focus your efforts on the most promising first, speeding your time to publication or the clinic.
Arrayed Edit-R CRISPR-Cas9 libraries provide:
Pooled screening libraries enable you elucidate biological pathways and genes of interest in highly multiplexed experiments, thereby limiting the equipment and materials needed to perform a successful screen. Enabling delivery to almost any cell type, even those refractory to transfection, lentiviral particles provide a means to select for only the cells that have been transduced, ensuring you are working in a stable population with minimal background.
Pooled Edit-R lentiviral sgRNA libraries provide:
When choosing a CRISPR-Cas9 screening platform, there are many considerations in determining the best tools for your experimental needs. Instrumentation, analytical support, and assay type(s) must all be taken into account.
Pooled sgRNA or arrayed crRNA for high-throughput gene editing studies
Webinar with examples of functional analysis assays for arrayed screening and results from a high-content screen.
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