Will an siRNA designed to target the human version of a particular gene work in a different species?

siRNAs designed specifically to target human genes are not expected to silence homologs in other species. Thus far, of the siRNAs designed for a gene in a particular species, few cross-target another species, even in cases where a high identity exists between the gene sequences of two species. There are instances where siRNAs can be specifically designed to be functional in two or more species, but this involves careful siRNA design and bioinformatics analysis. The online siDESIGN Center provides the necessary tools to support cross-species design requests. Contact Dharmacon Technical Support (Ts.dharmacon@horizondiscovery.com) for more information.

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