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Why am I seeing green cells when inducing expression with my TRIPZ construct?
This may be due to bleedthrough. If the cells with the TRIPZ construct are so bright with the red fluorescence from the TurboRFP the green filter may not be able to filter out all the fluorescence emitted at the 574nm wavelength and the cells will appear green. Test to see if the brightest red cells are also the ones that appear green.