What are recommended purification methods for 5'-modified RNA oligos?
Modified bases or labels added during solid-phase synthesis, such as most 5'-modifications, typically do not require further purification. Purification is recommended for modifications or labels added post-synthetically. Modified or labeled RNAs are generally purified by HPLC purification which we can provide as an optional service. Additionally, it is highly recommended that any purification steps be performed on 2'-ACE protected RNA oligos since the protecting groups help limit possible secondary structure, resulting in a tighter band and better purification.