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What is the recommended primer for sequencing Human ORF clones in the pDONR223 vector?
Sequencing primer for the insert in pDONR223 (found in product insert):
5' - TTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGT - 3'
Also, there is a 20bp match between the T7 promoter and the "reverse primers" region of pDONR223. The so-called "T7 short" primer (http://cancer-seqbase. uchicago.edu/primers.html) sequence (TAATACGACTCACTATAGGG) matches perfectly. There are no T7 or SP6 promoters.