What production method and quality control testing is performed for synthetic sgRNA?

Edit-R synthetic sgRNA is synthesized using GE Healthcare Dharmacon 2’-ACE chemistry, purified by size exclusion, deprotected, and desalted. The synthetic sgRNA is then identity confirmed by MALDI-TOF mass spectrometry and is quantified by readings at A260 using a spectrophotometer. Finally, the appropriate quantity is dried down and packaged in a 1.5 mL tube or 96-well plate. For different purification options and larger amounts of synthetic sgRNA these can be custom ordered through the single stranded RNA synthesis tool.

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