Home | Resources | FAQs

What is the difference between the SMARTpool, the Set of 4, and the Set of 4 Upgrade?

These products are three different ways of purchasing the same four duplexes. A SMARTpool reagent is a mixture of four siRNAs (all designed to target separate regions of the single gene of interest) and comes with a performance guarantee of at least 75% silencing under standard cell culture conditions (guarantee applies to siGENOME and ON-TARGETplus only).* The four duplexes are pooled together and supplied in a single tube. Sequence information is provided with all SMARTpool purchases. The Set of 4 product format provides separate aliquots of each of the four duplexes and thus is supplied in four separate tubes. Sequence information accompanies this product along with a performance guarantee that at least three of the four supplied duplexes will silence target gene expression by 75% or more when used under standard cell culture conditions (guarantee applies to siGENOME and ON-TARGETplus only).* The SMARTpool Set of 4 Upgrade is only available if the SMARTpool reagent for the same gene product has been previously purchased. The Upgrade provides separate aliquots of each of the four duplexes and thus is supplied in four separate tubes. Sequence information accompanies this product along with a performance guarantee that at least three of the four supplied duplexes will silence target gene expression by 75% or more when used under standard cell culture conditions (guarantee applies to siGENOME and ON-TARGETplus only).* * The performance guarantee applies when silencing is monitored at the mRNA level 24-48 hours after transfection using 100 nM siRNA. Optimum transfection efficiency must be confirmed using one of Dharmacon's positive control siRNA duplexes or another previously validated functional siRNA duplex. Low transfection efficiency may result in a lower level of silencing. Transfection efficiency may vary between different cell lines and should be optimized for each cell line used.

Related Categories: