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What cell line do I need to use for lentiviral packaging?

You can use any 293T cell line to package lentivirus. You do not have to use the HEK293T cell line included with the Trans-Lentiviral Packaging System to package lentivirus. The key element that your cell line needs to contain is the Large T antigen. 293T cells have been altered to express the Large T antigen from SV40 virus. The main function of the Large T antigien is to help the plasmids with an SV40 origin of replication to replicate and be retained in the cell after a tranfection. This will in turn allow the cell to make more of the virus during packaging. We are unable to help trouble shoot if you choose to package with any other cell line but the HEK293T, but that does not mean it will not work.

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