Can I use the same SMARTchoice promoter to express the shRNA in multiple cell lines?

As a general rule, we recommend using the SMARTchoice shRNA Promoter Selection Plate to identify the promoter with the highest activity in each cell line or cell type. Promoter strength is important to the success of vector-based gene silencing, which can vary widely across cell lines and among species. The SMARTchoice shRNA Promoter Selection Plate is designed to identify the optimal promoter specific for each cell line or cell type.