Can I use the Edit-R synthetic tracrRNA and crRNA components with my own preferred Cas9 nuclease expression plasmid?

We have validated the use of Edit-R synthetic tracrRNA and crRNAs and achieved efficient gene editing utilizing the Edit-R Cas9 expression plasmids in mammalian cell lines. Thus we cannot predict the efficacy of, nor can we troubleshoot experiments performed with, any other Cas9 nuclease expression vector. However, the repeat component of the crRNA sequence and the entire tracrRNA sequence are derived from the Streptococcus pyogenes CRISPR-Cas9 system, so they very likely can successfully be used with another S. pyogenes Cas9 nuclease expression plasmid, as long as the expressed Cas9 sequence is suitably codon-optimized and is under the control of a promoter which is active in your cells of choice. Additionally, you must be able to efficiently co-transfect your plasmid DNA with the synthetic RNAs.

Share: