Can RPMI media be substituted for the DMEM media listed in the culturing protocol for the HEK293T cells?

We do not recommend that you use the RPMI media unless for some experimental reason you have to use it. The two medias do not contain the same components and our HEK293T cells are acclimated to the DMEM media listed in the culturing protocol in the Trans-Lentiviral Packaging System manual.