Are ON-TARGETplus siRNA guaranteed to have fewer off-target effects?

We believe that the 3-pronged strategy (design, modifications, and pooling) used to create ON-TARGETplus siRNA is the best available means of reducing off-target silencing. We have seen up to 90% of off-targets eliminated with this strategy. However, due to the complex nature of the pathway and the difficulty in identifying or quantifying true off-targets, we do not offer a guarantee in this area.