How can I get more 5X siRNA Buffer?

The 5X siRNA Buffer is available for purchase on our web site. Alternatively, under RNase-free conditions, you may prepare additional 5X siRNA Buffer. Composition: 300mM KCl, 30mM HEPES-pH 7.5, 1.0mM MgCl2 Consumables: 1.100 mL sterile bottle or flask 2.15 mL conical tube Chemicals: 1.RNase-free water 2.Potassium chloride 3.HEPES-free acid 4.Magnesium Chloride 6H2O 5.Potassium Hydroxide pellets Procedure: 1.Prepare annealing buffer in a sterile 100mL tube a.30 mL RNase-free water b.2.24 g potassium chloride c.0.72 g HEPES-free acid d.0.02 g magnesium chloride 6H2O 2.Prepare fresh 2.0 M KOH in a separate 15 mL conical tube a.1.20 g potassium hydroxide pellets b.10.0 mL of RNase-free water 3.Add 2.00 mL of the freshly prepared 2.0 M KOH to the annealing buffer from step 1 4.Add sterile RNase-free water and mix to a fi nal volume of 100.0 mL 5.Aliquot 1.0 mL for pH testing. The pH should be between 7.3-7.6 6.Sterile filter or autoclave the solution 7.Store the solution at 4°C for 3 months

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