How many freeze-thaw cycles can the Edit-R Cas9 Nuclease mRNA, tracrRNA and crRNAs be subjected to?

We recommend not exceeding two to three freeze-thaw cycles for Cas9 mRNA, and four to five freeze-thaw cycles for tracrRNA and crRNA to ensure product integrity. On initial use, thaw the Cas9 mRNA on ice and aliquot remaining stock into convenient volumes and store all aliquots at -80 °C to minimize the number freeze-thaws cycles prior to each experiment.