Can the Edit-R system be used for gene knockout in non-mammalian organisms, such as bacteria or worms?
We have designed the Edit-R Cas9 plasmids for expression (and thus have only tested) in mammalian cells. Thus we cannot predict the efficacy of using Edit-R synthetic RNA components, nor can we troubleshoot experiments performed in non-mammalian systems. However, the repeat component of the crRNA sequence and the entire tracrRNA sequence are derived from the Streptococcus pyogenes CRISPR-Cas9 system, so they very likely can be used with another S. pyogenes Cas9 nuclease expression plasmid, as long as the expressed Cas9 sequence is suitably codon-optimized and is under the control of a promoter which is active in your cells of choice. Additionally, you must be able to efficiently co-transfect your plasmid DNA with the synthetic RNAs.