Can I co-transfect the Edit-R synthetic crRNA and tracrRNA components with the Edit-R Lentiviral Cas9 Nuclease that I bought as plasmid DNA rather than making lentiviral particles?

The Edit-R Lentiviral Cas9 Nuclease Expression vectors are intended to be used as lentiviral particles to generate stable cell lines expressing Cas9. If you intend to perform genome engineering by transient transfection, we recommend the non-lentiviral Edit-R Cas9 Nuclease Expression plasmids, which are smaller in size and intended for co-transfection with the synthetic RNAs.

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