Can I co-transfect the Edit-R crRNA:tracrRNA components with the Edit-R Lentiviral dCas9-VPR expression plasmid?

We do not recommend doing a co-transfection of Edit-R Lentiviral dCas9-VPR Expression plasmids with synthetic guide RNAs due to the transient nature of transcriptional activation with the non-expressed guide RNAs and the longer time necessary to perform blasticidin selection (typically > 5 days) to remove the non-transfected cells. For transient expression experiments, or to avoid the use of lentiviral particles, we recommend doing a co-transfection of the Edit-R sgRNA plasmid with the Lentiviral dCas9-VPR expression plasmid which then allows for selection of both components.