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    CRISPRa for Transcriptional Activation

    Synthetic & lentiviral reagents for endogenous gene overexpression

    Dharmacon Edit-R CRISPR activation (CRISPRa) reagents provide a straightforward, efficient set of tools to study a gene’s function by overexpression in its native context. Our predesigned reagents for human and mouse genes make it easy!

    CRISPR-Cas9 is not only for creating genomic double strand breaks, it can also be used to target promoter regions to activate or inhibit transcription. Using a deactivated or dead Cas9 nuclease fused to transcription activators, like dCas9-VPR, in combination with a guide RNA in either a synthetic or lentiviral format, the endogenous expression of a gene can be up-regulated by anywhere from 5 to 50,000+ fold!

    Explore our CRISPRa products for transcriptional activation

    • Edit-R CRISPRa crRNA Libraries

      Arrayed collections of synthetic CRISPR RNA for overexpression screening of gene families, druggable, and whole human genome.

    • Edit-R CRISPRa Guide RNA

      Algorithm-designed lentiviral and synthetic reagents specifically target promoter regions of human and mouse genes to induce a gene’s transcriptional activation.

    • Edit-R dCas9-VPR

      Lentiviral particles or purified plasmid that express nuclease-deactivated Cas9 fused to transcriptional activators. When complexed with a guide RNA will trigger an endogenous gene’s expression.

    • Edit-R CRISPRa Positive crRNA Controls

      Pooled or individual crRNA controls for assessment of optimal experimental conditions for gene activation.

    • Edit-R CRISPRa Non-targeting crRNA Controls

      Non-targeting controls to evaluate cellular responses to CRISPRa components in the absence of gene target-specific crRNA.

    • Edit-R CRISPRa Lentiviral sgRNA Positive Controls

      Control sgRNAs targeting well-characterized genes to determine the effectiveness of your experimental conditions for maximum activation.

    • Edit-R CRISPRa Lentiviral sgRNA Non-targeting Controls

      Non-targeting controls to evaluate cellular responses to CRISPRa components in the absence of target-specific sgRNA.

    • Cherry-pick libraries

      Upload your own gene list to customize and order plates of predesigned crRNA for CRISPRa studies across tens or thousands of genes.

    Edit-R CRISPRa products may be combined in several ways to support your particular experimental needs. Whether you’re doing co-transfection or creating a stable dCas9-VPR cell line (recommended), choose from our synthetic RNA, lentiviral particles, or glycerol stock (plasmid) sgRNA, and combine with dCas9-VPR plasmid or lentiviral particles.

    Resources for CRISPR Activation

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  • Webinar: CRISPRa tools for transcriptional activation studies