Synthetic & lentiviral reagents for endogenous gene overexpression
Dharmacon Edit-R CRISPR activation (CRISPRa) reagents provide a straightforward, efficient set of tools to study a gene’s function by overexpression in its native context. Our predesigned reagents for human and mouse genes make it easy!
CRISPR-Cas9 is not only for creating genomic double strand breaks, it can also be used to target promoter regions to activate or inhibit transcription. Using a deactivated or dead Cas9 nuclease fused to transcription activators, like dCas9-VPR, in combination with a guide RNA in either a synthetic or
lentiviral format, the endogenous expression of a gene can be up-regulated by anywhere from 5 to 50,000+ fold!
Explore our CRISPRa products for transcriptional activation
Arrayed collections of synthetic CRISPR RNA for overexpression screening of gene families, druggable, and whole human genome.
Algorithm-designed lentiviral and synthetic reagents specifically target promoter regions of human and mouse genes to induce a gene’s transcriptional activation.
Lentiviral particles or purified plasmid that express nuclease-deactivated Cas9 fused to transcriptional activators. When complexed with a guide RNA will trigger an endogenous gene’s expression.
Pooled or individual crRNA controls for assessment of optimal experimental conditions for gene activation.
Non-targeting controls to evaluate cellular responses to CRISPRa components in the absence of gene target-specific crRNA.
Control sgRNAs targeting well-characterized genes to determine the effectiveness of your experimental conditions for maximum activation.
Non-targeting controls to evaluate cellular responses to CRISPRa components in the absence of target-specific sgRNA.
Upload your own gene list to customize and order plates of predesigned crRNA for CRISPRa studies across tens or thousands of genes.
Edit-R CRISPRa products may be combined in several ways to support your particular experimental needs. Whether you’re doing co-transfection or creating a stable dCas9-VPR cell line (recommended), choose from our synthetic RNA, lentiviral particles, or glycerol stock (plasmid) sgRNA, and combine with dCas9-VPR plasmid or lentiviral particles.