The Yeast Synthetic Histone H3 and H4 Mutant collection consists of systematic residue substitutions totaling 486 mutants. Each of these is shown mapped here.
Cassettes contain synthetic H3 and H4 genes (HHTS and HHFS) flanking a central native HHT2/HHF2 promoter region (PHHT2-HHF2). Mutations are engineered into either HHTS or HHFS and tagged with molecular barcodes (TAGs, labeled *). Upper cassette indicated is used as base construct for HHTS (H3) mutants; lower one is used as base construct for HHFS (H4) mutagenesis.