• banner: Edit-R CRISPR-Cas9 Genome Engineering

    Edit-R CRISPR-Cas9 Gene Knockout, CRISPRa, and HDR Products

    Optimized tools for high-confidence genome engineering

    The Dharmacon Edit-R CRISPR-Cas9 platform provides guaranteed, predesigned, ready-to-use guide RNAs to enable rapid and highly functional gene editing experiments.

    • Higher assurance of functional knockout or transcriptional activation from predesigned genomewide guide RNAs
    • Improve expression of dCas9-VPR or Cas9 nuclease by choosing an optimal promoter for your cell type
    • High-quality synthetic and lentiviral reagents to meet every experimental need
    • Licensed from the Broad Institute as provider of CRISPR reagents for research use

    Synthetic Guide RNA

    • Edit-R predesigned crRNA

      Guaranteed to edit your target! Algorithm-optimized crRNA for genome-wide coverage of human, mouse, or rat genes. Modifications for nuclease resistance improve DNA-free editing. Simply search for your gene!

    • Edit-R CRISPRa synthetic crRNA

      Offering coverage of the human and mouse genomes, these synthetic RNAs are modified for nuclease resistance and available as pools of four or individual crRNA reagents.

    • Edit-R tracrRNA

      Edit-R trans-activating CRISPR RNA (tracrRNA) is synthetic, HPLC-purified, long RNA required for use with Edit-R crRNA to form the complex that programs Cas9 nuclease. It is modified for nuclease resistance and can be used with modified or unmodified Edit-R crRNA.

    • Edit-R Synthetic sgRNA

      Custom synthetic 100-mer single guide RNA, input your own design or use our flexible design tools

    • Edit-R Synthetic Positive crRNA Controls and Detection Primers

      Species-specific crRNAs targeting well-characterized genes, as well as mismatch detection assay primers, to determine the effectiveness of your gene editing conditions for maximal efficiency.

    • Edit-R Synthetic crRNA Non-targeting Controls

      Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene target-specific crRNA.

    • Edit-R CRISPRa Positive crRNA Controls

      Pooled or individual crRNA controls for assessment of optimal experimental conditions for gene activation.

    • Edit-R CRISPRa Non-targeting crRNA Controls

      Non-targeting controls to evaluate cellular responses to CRISPRa components in the absence of gene target-specific crRNA.

    • Edit-R crRNA Libraries

      Pre-defined collections of popular gene families and pathways for high-throughput applications.

    • Edit-R CRISPRa crRNA Libraries

      Arrayed collections of synthetic CRISPR RNA for overexpression screening of gene families, druggable, and whole human genome.

    • Cherry-pick libraries

      Have a favorite gene list? Customize and order plates of predesigned crRNA for knockout studies in your targets of interest.

    Lentiviral Guide RNA

    Vector-based Cas9 Nuclease

    • Edit-R Cas9 Nuclease Expression Reagents

      Non-lentiviral vectors provided as endotoxin-free purified DNA for direct co-transfection with Edit-R synthetic crRNA and tracrRNA.

    • Edit-R Lentiviral Cas9 Nuclease reagents

      Purified lentiviral particles or plasmid DNA for generation of stable Cas9 nuclease-expressing cell populations. Constitutive or inducible promoter options are available.

    • Edit-R dCas9-VPR

      Lentiviral particles or purified plasmid that express nuclease-deactivated Cas9 fused to transcriptional activators. When complexed with a guide RNA will trigger an endogenous gene’s expression.

    DNA-free Cas9 Nuclease

    Custom Guide RNA Design & Ordering Tools

    • CRISPR Design Tool

      Place a custom guide RNA order, or design and order your own synthetic sgRNA, crRNA, or lentiviral sgRNA with our easy-to-use interface.

    HDR Donor Template Design & Ordering Tools

    HDR Donor Template Kits

    100% of Edit-R predesigned guide RNA are guaranteed to edit!

    Edit-R predesigned crRNA and lentiviral sgRNAs are guaranteed to edit your target, or we will replace it! No restrictions on Cas9 nuclease formats – if your Edit-R positive control works, so will your gene-specific guide RNA.

    The Edit-R Predesigned Guide RNA Guarantee

    We guarantee that EVERY predesigned Edit-R CRISPR-Cas9 crRNA and Edit-R lentiviral sgRNA (guide RNAs) will provide successful editing at the target site when delivered as described in the Edit-R Technical Manuals.

    The Edit-R guide RNA guarantee is valid when used with any wild type S. pyogenes Cas9 nuclease, including mRNA, expression plasmid, protein, or stable Cas9 expression, and Edit-R crRNAs must be used with Edit-R tracrRNA for the guarantee to apply.

    Analysis of editing of the treated cell population must be shown using a T7E1 or Surveyor mismatch detection assay. If successful editing is not observed for a predesigned Edit-R guide RNA while an appropriate side-by-side Edit-R positive control is successful, a one-time replacement of a different predesigned Edit-R guide RNA of the same format and quantity will be provided at no cost.

    A replacement will only be approved upon discussion with our Technical Support team.

    Successful editing at the DNA level does not always lead to functional gene knockout; it is recommended to test multiple guide RNAs to determine the most effective guide RNA for knockout of your target gene.

    This guarantee does not extend to any accompanying experimental costs, does not apply to guide RNAs ordered via the CRISPR Design Tool, and will not be extended to the replacement guide RNA.



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